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Deflasking
De-mystified
There
is no "magic" formula for deflasking seedlings but, because of
the frequent requests for information, we have prepared the following
information sheet. This is NOT the right way to do it BUT it is the way
that we do it for a home or low humidity environment AND IT WORKS FOR
US.
The
first thing to do is to ensure that the plants are ready to come out. If
the flask is contaminated, check it carefully every 12 hours and see if
it is spreading. If the contamination is spreading, the plants MUST come
out – NOW. If there is no contamination, leave the plants in the
flask until they are ready to come out. They are ready to come out when:
1)
Ideally, most of the plants should have plump, healthy roots and at
least 2 leaves, each of which is about an inch or two or so. (If they
are not that large, consider finding another source.) If they are
not that large, leave them in the flask somewhere where they receive
some light. They will continue to grow. If you leave them in the
sun, they will burn.
2)
If the medium begins to dry out (which happens when there is not enough
medium initially), you might as well take out the plants because they
will not grow in the flask. (Another reason to find another source
for your flasks.)
Flasks
come in 2 types: glass and plastic. The plants can be taken out gently
(or you can at least attempt to) or the glass can be broken and the
plastic cut.
To
take them out GENTLY: Add lukewarm water to the flask 2/3 of the way.
Hold your hand over the open end and shake the flask to loosen the agar.
Pour out the water and agar (into a container – in case a plant falls
out. If not, pour out the water.). Repeat until there are only plants
and no more agar. Take out the plants carefully. You can take them out
several different ways. 1) You can get a "long" piece of metal
and curve the end and "help" the plants to come out. Careful!
You can break off roots. 2) Put in 2 fingers and grasp a leaf. Careful!
You can pull out the centre leaf of a plant.
In
both cases, after a few plants have been removed, it will be easier to
take out the rest.
To
break glass: The easiest way is
to tape masking tape over the whole bottle (including on the bottom)
leaving an untaped ring around the bottom circumference of the bottle.
Wrap the bottle in paper towels. Hit the flask with a hammer where there
is no tape. Your seedlings and the agar can be taken out. BE CAREFUL.
THERE ARE SHARDS OF GLASS.
Plastic
flasks:
You will have to cut these with something really sharp but BE CAREFUL
because you can cut the seedlings as well.
In
either case, you now have the seedlings and the agar. You have to wash
the agar off the seedlings, preferably in some water with a small amount
of fungicide.
We
now have all the seedlings from the flask (one way or the other). I
believe that sphagnum moss is the best medium for seedlings from flask.
I get a 4½" pot, put some styrofoam in the bottom for drainage.
Then, like farmers planting their crops in rows, I put a row of moss on
one side of the pot. Then I take the seedlings one by one and place as
many seedlings as I can fit in one row. I place the lowest leaf just
above the moss. I hold them in place while I put in a second row of
moss. Another row of seedlings and another row of moss. A third (final)
row of seedlings and a last row of moss. You will end up with 4 rows of
moss and three rows of seedlings.
I
leave the compot out in the room air for an hour or so – until the
leaves START to go limp at the tips. (Depending on the roots, the
ambient humidity, etc., this could take more or less than 1 hour.)
Then
I place the compot in a ziploc bag. I do not ziploc it, just fold it
over. The next day, I leave the bag open for an hour or until the tips
start to wilt. The day after that, the bag is left open for 2 hours and
so on for about a week. (N.B. The bag is NEVER sealed, only folded
over.) At the end of a week or so, the plants are hardened off. I leave
them in the bag but I fold the top of the bag outward so that there is
good air circulation.
The
plants are re-potted after 4 (yes, that’s right 4!) months and
re-potted again 4 months after that. After the first year, they should
be near blooming size (6-10" leafspread) and many will bloom within
the first year.
I
have had "hands-on" deflasking sessions at different orchid
societies and, a few months later, people come up to me and tell me how
wonderfully their plants are doing.
GOOD
LUCK AND GOOD GROWING!
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SIB
CROSSES
What are they?
When
the pollen of one plant ("the father") is placed on the
stigmatic surface of another plant ("the mother") and seed
results, a new hybrid is created. The seedlings produced are often
referred to as the F1 (or first) generation.
Generally,
if every one of the plants was bloomed, the results could be plotted and
would result in a bell curve. With the average plant being on the
highest point of the curve (dead centre) and the really bad and the
really good plants being at the two extremes. Often a breeder will take
an excellent plant and self it (breed it to itself – yes, Virginia,
plants can do that) or take two plants from this first generation and
breed them to each other. Such breeding is referred to as a selfing (in
the first case) or a sib (or sibling) cross (in the second case). In
either case, the resulting seedlings are called the F2 (or second)
generation. This process can be ongoing for as many generations as
anyone wants.
A
very good example of this is Phal. Golden Buddha. The original cross was
Phal. Cher Ann x Phal. Spica. For their day, the F1 flowers were
different, highly coloured and reasonably well shaped for that time. In
an effort to improve the shape and keep the intense coloring, sib
crosses were done by many people including Stewart’s Orchids, Coqui
Nurseries, Gemstone Orchids and us. We are now at an F7 or F8.
By
selective breeding, an attempt is made to concentrate the gene pool,
keeping only those genes that are on the "better" side of the
bell curve. Although this is awfully simplified, we can say that
genes come in two main types: good and bad genes. The good
genes give us flat flowers, the bad ones cup or reflex the flowers.
The good genes produce inflorescences that are the right length
with flowers that are well shingled, the bad ones give short or long
inflorescences with poorly presented flowers. Good genes produce
vibrant, distinct colours, the bad ones produce dull or muddy colours.
Obviously,
sib crosses and selfings can also produce flowers that "only a
mother could love" but due to the increased chances of getting a
superb result, many growers keep trying sib crosses. Once you've
grown one, you'll be back for more.